This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie COFUND grant agreement No 665735.

Bio4Med Research Projects:


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1: Bio4Med - Epigenetic mechanisms associated with reprogramming of tumor infiltrating microglia/macrophages into pro-tumorigenic cells.

Supervisor:
prof. Bozena Kaminska-Kaczmarek
Foreign partner:
Prof. Helmut Kettenmann, Max Delbruck Center, Molecular Neurosciences, Berlin, Germany
WWW
http://en.nencki.gov.pl/laboratory-of-molecular-neurobiology
Background:
Currently, there is no data regarding transcriptional and epigenetic regulators of long-lasting changes in gene expression during microglia polarization in animal models of gliomas. Our preliminary studies demonstrated alterations of epigenetic modification patterns in correlation with profound changes in gene expression suggesting the role of epigenetics in long-term changes in microglia polarization. We plan to analyze histone modification and DNA methylation patterns in a whole genome scale and to identify genes regulated by those modifications. We will perform a combined analysis of chIP-seq for selected activating and repressive modifications of histones and RNA seq in microglia/macrophages sorted from experimental and human gliomas. Maps of active/repressed chromatin regions will be intersected with maps of transcription factor binding sites using computational tools. Spatial localization of selected genes within the nucleus will be analyzed by FISH and immunocytochemistry. The most interesting interactions and findings will be studied in knockout mice models.
Aim:
The main aim of the proposed research is to identify transcriptional and epigenetic mechanisms shaping immune microenvironment of gliomas and affecting anti-tumor immunity. Glioma cells release factors which induce pro-tumorigenic (M2-like) activation of tumor infiltrating microglia that results local and systemic immunosuppression.
Requirements:
We expect a good master (or equivalent) degree (minimum upper second) in a relevant discipline of the life sciences (preferentially neurosciences, immunology, vascular biology, molecular or cellular biology) and good skills in English language. Research experience and practice with animal models would be advantageous. Each PhD student will be expected to have at least one second placement (3-6 months) to collaborating partner organization. The successful applicants will be expected to register for a PhD and will submit a high quality dissertation for examination within 3 years of appointment. Candidates must not have resided or carried out their main activity (work, studies, etc.) in Poland for more than 12 months in the past 3 years.