This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie COFUND grant agreement No 665735.

Bio4Med Research Projects:


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2: Bio4Med - Profiling microRNA expression in lymphocytes from patients with early Alzheimer's disease: search for disease mechanisms and early biomarkers

Supervisor:
prof. Urszula Wojda
Foreign partner:
Prof. Angeles Martin Requero, Centro de Investigaciones Biologicasv, Madrid, Spain
WWW
http://en.nencki.gov.pl/laboratory-of-preclinical-testing-of-higher-standard
Background:
The PhD student taking part in this project will gain insight into modern concepts of complex multifactorial diseases such as AD, as well as receive training in molecular and cell biology techniques such as isolation from blood, cell culture and flow cytometry analysis of patients’ lymphocytes, isolation of miRNA, quality control and RT-PCR profiling assays, bioinformatic analysis of the high throughput data, cell transfections and miRNA-based silencing of target genes, immunoblotting, cytology and confocal microscopy.
Aim:
The project aims at identifying the miRNA signature in early and late stages of Alzheimer’s disease (AD) in human lymphocytes, as well as identifying corresponding miRNA-regulated proteins and signaling pathways. Therefore, this study can provide novel insight in the early molecular processes responsible for AD development, help to establish the sequence of molecular changes during AD development, and to indicate early novel therapeutic targets in AD. Moreover, identified miRNAs could serve as novel early AD biomarkers that can be detected using relatively inexpensive methods in easily accessible blood cells.
Requirements:
Master degree within biological or related sciences with a strong background in molecular biology, cell biology and/or biochemistry; Good English language skills, both written and spoken; High motivation for the planned research, creativity, organizational skills, teamwork skills Advantage: experience in flow cytometry, confocal microscopy or bioinformatics (an advantage, but not a requirement. Candidates must not have resided or carried out their main activity (work, studies, etc.) in Poland for more than 12 months in the past 3 years.