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Bio4med | Research Projects

This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie COFUND grant agreement No 665735.

Bio4Med Research Projects:


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9: Bio4Med - The role of ZF5 motif in the regulation of genes with circadian rhythm disrupted in epileptic animals
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Supervisor:
prof. Katarzyna Lukasiuk
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Foreign partner:
Prof. Christophe Bernard,Institut National de la Sante et de la Recherche Medicale, Marseille, Franc
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WWW
http://en.nencki.gov.pl/laboratory-of-epileptogenesis
Background:
Recently both laboratories in cooperation demonstrated circadian oscillations of large ensembles of genes in the hippocampus. Preliminary in silico analysis of promoters of genes with similar expression patterns revealed common features.
Aim:
The aim of the project is further in silico analysis of regulatory regions of genes with similar circadian expression patterns and experimental verification of potential role of the binding of transcription factors, e.g. to ZF5 binding site to promoters of candidate genes. In silico analysis will be done using in house methods developed at Nencki. Occupancy of promoters will be determined by chromatin immunoprecipitation (Chip) followed by qPCR. Localization of transcription factors will be determined by immunohistochemistry.
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Requirements:
Candidates should have a recent Master degree (or equivalent) in biological sciences, medicine, bioinformatics or related fields. Good command of English is required. Interdisciplinary character of the project requires form the candidate willingness to learn new methods. An experience in animal models of diseases, bioinformatics, molecular biology, microscopy and/or histology will be an advantage. Candidates must not have resided or carried out their main activity (work, studies, etc.) in Poland for more than 12 months in the past 3 years.

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